Fig. 8
From: Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus
PELI1 expression is negatively correlated with SLE disease activity. a, b Flow cytometric analysis of the frequencies of CD19−CD138+ plasma cells in peripheral blood mononuclear cells (PBMCs) from health donors (HD) and SLE patients. Data are presented as a representative plot (a) and summary graph (b). c–e Correlation analysis of the PELI1 mRNA expression in PBMCs with the disease activity (c), plasma cells frequencies (d), and serum IgG levels (e) in SLE patients. f Heat map showing differentially expressed genes in PBMCs that isolated from SLE patients with Peli1high or Peli1low mRNA expression (SLE-PH or SLE-PL). g Volcano graphs showing the genes differentially expressed in PBMCs between SLE-PH and SLE-PL. h QPCR determining the relative expression of the indicated apoptosis-related and Peli1 genes in PBMCs of SLE-PH and SLE-PL patients. Each dot represents a value of the gene epression results from SLE-PH or SLE-PL patients. Data were normalized to a reference gene, ACTIN. Data are shown as the mean ± SEM based on three independent experiments. Two-tailed Student’s t-tests were performed. *P < 0.05 and **P < 0.01
