Fig. 3

Nucleolar stress triggered by the perturbation of ribosome biogenesis leads to excessive lipid accumulation. a The general processes of ribosome biogenesis. The fat content percentage of triacylglycerol (% TAG) in total lipids (TAG + phospholipids, PL) of each worm strain was quantitated using TLC/GC analysis. Data are presented as the means ± SD of three biological repeats. b Ribosomal profiles of WT, rrp-8(kun54), rsks-1(ok1255), and AD-treated worms. c Quantification of the polysomes. The areas under each peak were calculated using ImageJ software. The fold-change of each peak size for a specific worm strain is presented compared to the 80S peak size of wild type (WT). Data are presented as the means ± SD of three biological repeats. d, f Nile Red staining of fixed worms. Representative animals; the anterior is indicated on the left, and the posterior is indicated on the right. Scale bar represents 20 μm. e, g Distribution of the lipid droplet size (% lipid droplets), as measured by Nile Red staining of fixed worms from d and f, respectively. Data are presented as the means ± SD of 10 animals for each worm strain. h Percentage of triacylglycerol (% TAG) in total lipids (TAG + phospholipids, PL) analyzed by TLC/GC. Data are presented as the means ± SD of four biological repeats. Significant difference between a specific worm strain and its genetic background strain, Student’s t-test, ***P < 0.001, **P < 0.01, *P < 0.05. NS, no significant difference