Fig. 5

RPL-11.2 and RPL5 are required for nucleolar stress induced upregulation of PHA-4. a Confocal microscopy of PHA-4::GFP {wgIs37[pha-4::GFP]} in WT and rrp-8(kun54) worms at 1 day of adulthood under treatment of control (empty vector, EV), rpl-11.1, rpl-11.2, and rpl-5 RNAi from L2/L3 larvae stage, respectively. BF, bright field. Representative animals; the anterior is indicated on the left, and the posterior is indicated on the right. Scale bar represents 10 μm. b Quantification of the fluorescence intensity of PHA-4::GFP from a. Data are presented as the means ± SD of at least 20 worms for each worm strain. Significant difference between WT and rrp-8(kun54) mutant background, Student’s t-test, ***P < 0.001. c Efficiency of RNAi knockdown (KD). Relative mRNA expression of rpl-11.1, rpl-11.2, and rpl-5 was measured by QPCR in rrp-8(kun54) worms treated with its corresponding RNAi clone from the L2/L3 larvae stage. Data are presented as the means ± SD of three biological repeats. Significant difference between the control (empty vector, EV) and a specific gene of RNAi bacteria, Student’s t-test, **P < 0.01, *P < 0.05. d Nile Red staining of fixed WT, rrp-8(kun54) and rsks-1(ok1255) worms treated with empty vector (EV), rpl-11.2, and rpl-5 RNAi from the L2/L3 larvae stage. Representative animals; the anterior is indicated on the left and the posterior is indicated on the right. Scale bar represents 20 μm. e Percentage of triacylglycerol (% TAG) in total lipids (TAG + phospholipids, PL) analyzed by TLC/GC. Data are presented as the means ± SD of three biological repeats. Significant difference between a specific mutant strain without (EV) and with RNAi knockdown, Student’s t-test, ***P < 0.001. f Distribution of the lipid droplet size (% lipid droplets) measured by Nile Red staining of fixed worms from d. Data are presented as the means ± SD of 10 animals for each worm strain. Significant difference between a specific mutant strain without (EV) and with RNAi knockdown, Student’s t-test, ***P < 0.001