Fig. 2

Replication initiation landscape and dynamics in cells with 50% reduction in histone H1 levels. a Representative IGV snapshot showing the SNS-Seq coverage and identified ORIs from two biological replicates derived from 300 to 1500 nt SNS purified from wild-type (WT) mES (blue tracks) or H1-TKO mES cells (red tracks). Data from MEFs WT and HMGB1-KO SNS-Seq are also shown for comparison (green and orange tracks). b IODs, c fork rates and d percentage of fork asymmetry calculated from stretched fibres from WT and H1-TKO cells. See Supplementary Fig. 1g-j for full data analysis and numerical values. Data are pooled from three replicate experiments (n = 3). Box-plots definitions are as in Fig. 1h. e Protein levels of P-MCM2, MCM2, P-CHK1, CHK1, P-H2AX and H2AX at the cytosolic (C), nucleoplasmic (N) and chromatin (Chr) fraction of both cell types. The quantification of the phosphorylated band intensity normalized to the corresponding total band intensity for each sample is shown on the right. f IODs, g fork rates and h % of fork asymmetry upon treating both cell types with PHA-767491 (PHA) or ribonucleosides (RbNs) for 100 min. Median values are indicated (n = 2). Differences between distributions were assessed with the Mann–Whitney rank sum test. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. See Supplementary Fig. 4b for numerical values