Fig. 3

Replication-timing profiling of WT and H1-TKO mES cells. a Exemplary replication-timing profile of a large segment of Chromosome 10. Raw values for probe log ratios [log₂(Early/Late)] and the local polynomial Loess smoothing curve from two replicate experiments of mES WT (blue) and mES H1-TKO (red) are shown. b Enhanced view of the two regions marked in a showing an exemplary delayed and advanced timing region in H1-TKO cells. See Supplementary Data 1 for the genomic location and features of the 97 regions with altered replication timing in H1-TKO cells. Coverage analysis of GC content (c), gene density (d) and ORIs (e) in the early (E), middle (M), late (L), advanced (Adv) and delayed (Del) replication-timing domains (% of bp). Box-plots definitions are as in Fig. 1h. Median values are indicated by a black line and means by a red dot. Data not included between the whiskers are plotted as outliers (empty dots)