Fig. 5
From: Chromatin conformation regulates the coordination between DNA replication and transcription
RNAPII inhibition suppresses the replication phenotypes of H1-TKO cells. a Representative images and quantification of nuclear EU staining of WT and H1-TKO cells untreated or treated with a-amanitin for 6 h. Scale bar, 20 μm. b Distribution of S9.6 nuclear intensity ±RNAseH incubation in WT mES and H1-TKO mES cells untreated or treated with a-amanitin. Data are pooled from two independent experiments (n = 2). Median values are indicated. c IODs, d fork rates and e % of fork asymmetry calculated from stretched fibres of cells untreated or treated with a-amanitin. f Representative images of γH2AX foci (left) and distribution of γH2AX intensity per nucleus (right) in cells untreated or treated with a-amanitin. Scale bar, 10 μm. Median values are indicated. Data are pooled from two independent experiments (n = 2). Differences between distributions were assessed with the Mann–Whitney rank sum test. ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05. See Supplementary Fig. 7a-d for numerical values
