Fig. 4

Predicted TF motifs and electrophoretic mobility shift assay (EMSA) at the rs4776984 site indicate allele-specific binding. a Predicted TF motifs for CTCF and p300, as well as the hg19 reference genome sequence. b Biotinylated (labeled probe) 31-bp oligonucleotide complexes with ±15 bp flanking the reference or alternate allele for variant rs4776984 were incubated with nuclear protein extracted from primary HWA and resolved on a 6% polyacrylamide gel. Competitor assays were performed by incubating the reaction with ×100 excess of unlabeled (no biotin) oligonucleotide complexes with identical sequence. Arrow denotes specific binding of HWA nuclear protein to reference (left) and alternate (right) allele