Fig. 2 | Nature Communications

Fig. 2

From: Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment

Fig. 2

Characterisation of stimuli-responsive OmpF Panel. a EPR spectra of bis-(2,2,5,5-tetramethyl-3-imidazoline-1-oxyl-4-yl) disulphide-labelled OmpF-M experimental (black) and simulated (blue) and b bis-(2,2,5,5-tetramethyl-3-imidazoline-1-oxyl-4-yl) disulphide-labelled OmpF-M in 1% OG incubated with 10 mM DTT experimental (black) and simulated (blue). c Normalised FCS autocorrelation curves for SAMSA-CF in PBS (black), SAMSA-CF in 1% OG (blue) and OmpF-S-S-CF in 1% OG (Red). Dotted line—experimental autocorrelation curves, full line—fit. d SAMSA-CF release kinetics from OmpF-M in 30 mM GSH, 1% OG, as measured by FCS and analysed with a two-component fit. Error bars show standard deviations from 60 measurements

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