Fig. 1
From: MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation
MLL5 selectively suppresses RLR-mediated antiviral immune response. a Expression of Ifn-β, Tnf-α, and Il6 mRNA in BMDMs from wild-type (WT) or Mll5−/− mice stimulated with poly(I:C) (100 μg/ml), CpG-B (1 μg/ml), R848 (1 μg/ml), Pam3 (1 μg/ml) and LPS (0.2 μg/ml) for 4 h, or stimulated with intracellular poly(I:C) (1 μg/ml), intracellular 5′ppp-RNA (0.4 μg/ml) and intracellular ISD (1 μg/ml) for 6 h. Gapdh served as control. b Expression of Ifn-β, Tnf-α and Il6 mRNA in PMs from WT or Mll5−/− mice stimulated with intracellular poly(I:C) (1 μg/ml), intracellular 5′ppp-RNA (0.4 μg/ml) and intracellular ISD (1 μg/ml) for 6 h, or infected with VSV-GFP (MOI:1), SeV (10 HA/ml) and HSV-1 (MOI:1) for 6 h. Gapdh served as control. c ELISA quantification of IFN-β, TNF-α and IL6 secretion in PMs treated as in b. Data were from three independent experiments and were analyzed by Student’s t-test (two-tailed) and were presented as mean ± SD (*p < 0.05, **p < 0.01,***p < 0.001, ****p < 0.0001, ns means no significant difference)
