Fig. 4
Jedi1/2 and Yoda1 bind to the fragment of residues 1–2190. a, c, e, g Representative SPR traces of the immobilized purified mPiezo1 (top panel), mPiezo1(1–2190) (middle panel), and CED (lower panel) in response to the series of concentrations of the indicated compounds measured with the affinity mode. To validate the reproducibility of the immobilized proteins regenerated after each dose point, a same dose (5, 50, 250, and 373 μM for Yoda1, control compound, Jedi1, and Jedi2, respectively) was re-tested. As exemplified in e, the red line overlaps with the brown line, indicating the reproducibility of the immobilized proteins for the assay. b, d, f, h The resulting dose-binding curve of the indicated compounds to the indicated proteins. The curves in b, f, and h were fitted with a total-binding equation. Similar results were obtained from two to eight independent experiments
