Fig. 6
L1342 and L1345 are essential for Jedi1 and Yoda1 activation of mPiezo1. a, c Representative single-cell Fura-2 Ca2+ imaging traces showing the average responses of cells transfected with the indicated constructs in response to 1 mM Jedi1 (a) or 30 μM Yoda1 (c). b, d Dose response curves of cells transfected with the indicated constructs and GCAMP6s in response to Jedi1 (b) or Yoda1 (d). The curves were fitted with a Boltzmann equation. Each data point represents mean ± s.e.m., n = 4 wells. e Scatter plot of the chemical-induced currents from the Piezo1-KO-HEK cells transfected with the indicated constructs. Statistical significance was assessed using one-way ANOVA with multiple comparison test. f Pressure-current relationships of the stretch-induced currents of the L1342A/L1345A mutant under the indicated compound conditions. The curves were fitted with a Boltzmann equation. g Scatter plot of the P50 calculated from fit of the pressure-current relationship of individual recordings with a Boltzmann equation. Statistical significance was assessed using one-way ANOVA with multiple comparison test. Data shown as mean ± s.e.m., and the recorded cell number is labeled above the bar. *P < 0.05, ***P < 0.001
