Fig. 8
From: Divergent midbrain circuits orchestrate escape and freezing responses to looming stimuli in mice
Effects of selective inactivation of bilateral PBGN or LPTN on dimorphic defensive behaviors. a, b AAV-DIO-EGFP-2A-TeNT was locally injected into the bilateral PBGN (a, left) and LPTN (b, left) of vGlut2-ires-Cre mice, resulting in the specific expression of EGFP in glutamate+ neurons in the PBGN (a, right) and LPTN (b, right). Scale bars, 1.5 mm (left) and 30 μm (right) for (a) and (b). For single-channel micrographs and quantitative analyses, see Supplementary Fig. 7i and j. For validation of TeNT effect on neurotransmitter release, see Supplementary Fig. 8. c, f Distribution of locomotion speed during vs. before (left) and after vs. before (right) looming visual stimuli of mice with (TeNT) or without (Ctrl) inactivation of PBGN (c) and LPTN (f) neurons. d, g Distribution of LSIduring stimuli and LSIafter stimuli of mice with (TeNT) or without (Ctrl) selective inactivation of PBGN (d) and LPTN (g) neurons. e Quantitative analyses of the effects of PBGN inactivation on locomotion speed of mice exhibiting the Type II defensive behavioral pattern. h Quantitative analyses of the effect of LPTN inactivation on locomotion speed of mice showing the Type I defensive behavioral pattern. Data in (e, h) are means ± SEM (error bars). Numbers of mice are indicated in the graphs. Statistical analysis was Student’s t test (***P < 0.001; n.s. P > 0.1)
