Fig. 2

Primary mammary and pancreatic tumors systemically decrease cDC1s. a Representative flow cytometry gating strategy for mouse BM pre-DCs, CD24⁺ cDC1s, and Sirpα⁺ cDC2s. b Number of BM MDPs, CDPs, pre-DCs, immature granulocytes, granulocytes, CD24⁺ cDC1s, and Sirpα⁺ cDC2 from mice bearing end-stage orthotopic PyMT-B6 mammary tumors relative to tumor-free controls; n = 6/group. Data are representative of three independent experiments. c Number of BM pre-DCs, CD24⁺ cDC1, and Sirpα⁺ cDC2 in end-stage genetically engineered mouse models (GEMM) of BC (MMTV-PyMT) and PDAC (KPC) relative to tumor-free controls; tumor-free MMTV-PyMT controls n = 6; MMTV-PyMT, n = 6; tumor-free KPC controls, n = 7; KPC, n = 8. d Alternative representative gating strategy using Zbtb46-GFP mice continued from Fig. 2a for BM committed pre-cDC1s, including representative plots from tumor-free and PyMT-B6 tumor-bearing mice. Number of BM pre-cDC1s in Zbtb46-GFP⁺ mice bearing end-stage orthotopic PyMT-B6 mammary tumors relative to tumor-free Zbtb46-GFP⁺ controls; n = 6/group. Data are representative of two independent experiments. e Representative flow cytometry gating strategy for mouse blood pre-DCs. f Number of blood pre-DCs and granulocytes from mice bearing end-stage orthotopic PyMT-B6 mammary tumors relative to tumor-free controls; n = 6/group. Data are representative of three independent experiments. g Number of blood pre-DCs in end-stage genetic mouse models of BC (MMTV-PyMT) and PDAC (KPC) relative to tumor-free controls, n = 7/group. h Number of uninvolved LN CD103⁺ cDC1s from mice bearing end-stage orthotopic PyMT-B6 mammary tumors relative to tumor-free controls; n = 6/group. Data are representative of two independent experiments. i Mice with 1.0 cm diameter orthotopic PyMT-B6 tumors and tumor-free controls were implanted with matrigel plugs containing poly I:C+OVA-TxRd peptide in the upper mammary fat pad. Number of CD103⁺ cDC1s in the plug and draining LN and OVA-specific CD8⁺ T cells (CD3⁺CD8⁺Dextamer⁺) in the plug 10 days after implant; n = 6/group. Data are representative of two independent experiments. End-stage for each model is defined in the Methods. Error bars represent mean +/− s.e.m.; *p < 0.05, **p < 0.01, ***p < 0.001, n.s., not significant by unpaired two-sided Student’s t test