Fig. 7

GCSF regulates IRF8 in dendritic cell progenitors. a IRF8 measured in BM MDPs, CDPs, and pre-DCs of tumor-free mice, orthotopic PyMT-B6 tumor-bearing end-stage mice, and orthotopic PyMT-B6 tumor-bearing end-stage mice treated for 2 weeks with 50 μg anti-GCSF IgGs 3 × /week; n = 6/group. Data are representative of two independent experiments. End stage is defined in the Methods. b MPs, MDPs, and CDPs treated with 100 ng/ml GCSF and/or 100 ng/ml Flt3L for 24 h. Analyzed by RT-qPCR for Irf8 and Batf3. Data are representative of two independent experiments consisting of three wells per condition. c CD45.2⁺Lin^-Sca1⁻cKit⁺ MPs transduced with CD90.1 IRF8 overexpression vector or CD90.1 empty vector control were cultured on CD45.1⁺ BM feeder culture in the presence of 100 ng/ml Flt3L and/or 100 ng/ml GCSF for 3 days. Final cultures were analyzed for cDC1s (Live CD45.2⁺CD90.1⁺CD45.1^-MHCII⁺CD11c⁺Sirpα^-CD24⁺). Data are representative of three independent experiments consisting of three wells per condition. Error bars represent mean +/− s.e.m. or box plot; *p < 0.05, **p < 0.01, ***p < 0.001 by unpaired two-sided Student’s t test