Fig. 3
Distinct orientation on HIV-1 Env of BG18 compared to other V3/N332gp120 bNAbs. BG18 Fab (blue, surface) orientation on gp120 (gray, surface) overlaid with 10-1074 (a, magenta; PDB 5T3Z (http://dx.doi.org10.2210/pdb5T3Z/pdb)) and PGT122 (b, yellow; 5FYL (http://dx.doi.org10.2210/pdb5T3Z/pdb)) and PGT122 (b, yellow; 5FYL (http://dx.doi.org10.2210/pdb5FYL/pdb)) Fabs. c Overlay of CDRH3 loop of BG18 (blue), 10-1074 (magenta), PGT122 (yellow), PGT121 precursor (red; PDB 5CEZ (http://dx.doi.org10.2210/pdb5CEZ/pdb)), and PGT124 (orange; PDB 4R2G (http://dx.doi.org10.2210/pdb4R2G/pdb)) after alignment of bound gp120s (gray, surface) demonstrating a conserved structural motif recognizing the N332gp120 glycan (cyan). d, e Comparison of VH-VL domains of PGT122 (yellow) and 10-1074 (magenta) with BG18 (blue). The VH-VL domain orientation of BG18 on Env trimer is related by a 78˚ rotation and 6.8 Å translation about the indicated axis (black dashed arrow) to the 10-1074 variable domains after alignment against gp120 in the BG18-Env and 10-1074-Env structures
