Fig. 3
Inhibition of TNF-induced responses in neutrophils by TNFRI peptides. a, b Inhibition of superoxide production in neutrophils treated with TNF (1 ng/ml), which had been pre-treated with various concentrations of TNFRI209–211 or TNFRI206–211 for 30 min. c, d Lack of effect of scrambled peptides. e Inhibition of TNF-induced CL by TNFRI198–211. f, g Lack of effect of TNFRI209–211 and TNFRI206–211 on the fMLF-induced superoxide generation in neutrophils. The results are expressed as a % of fMLF-induced CL production. h, i Effect of TNFRI206–211 or TNFRI209–211 on TNF-rich medium (RM)-induced superoxide (chemiluminescence) production in neutrophils. TNF-RM (1:10 dilution in HBSS) was treated with TNFRI peptides and then tested for ability to stimulate neutrophil superoxide (chemiluminescence) production. Data represent the % inhibition of the TNF-RM-induced response. j The experiments in h were repeated with the D-form of TNFRI206–211. Data are presented as mean ± s.e.m. of the below-indicated number of experiments. Significance of difference between the presence and absence of peptide (Kruskal–Wallis test followed by Dunn’s multiple comparison test (n = 6 for a, n = 3 for b, n = 4 for c, n = 3 for d and f–i and n = 7 for e and j): *p < 0.05; **p < 0.01; ***p < 0.001; #p = 0.055
