Fig. 4
Basal-like human breast carcinomas display more centriole amplification than luminal breast carcinomas. a Representative immunofluorescence images of sections of human breast tumours showing normal centriole number (2) in interphasic cells of luminal breast carcinoma (upper left panel), and centriole amplification (>4), in both luminal (lower left panel) and basal-like (right panel) breast carcinomas. We labelled centrioles using the centriolar marker GT335, which labels tubulin glutamylation, a modification of tubulin present in centrioles, in co-localisation with the PCM marker, pericentrin. Tissue sections were also stained with DAPI (blue). Please note that we focused our analysis on interphase cells, as it is very difficult to find mitotic figures in breast tumours. Scale bar 5 µm, insets 1 µm. b Percentage of cells displaying centriole amplification in each of the 15 patients with luminal breast carcinomas (average 8% ± 2) and in each of the 10 patients with basal-like carcinomas ((on average 22% ± 2), p = 0.0004, Mann–Whitney test). Please note that only structures positive for both pericentrin and GT335 were taken into consideration to avoid false positives. All basal-like carcinomas (10 out of 10 patients) exhibited centriole amplification while it was not detected in 5 out of the 15 luminal carcinomas. Between 20 and 133 cells were analysed for each patient (see Supplementary Fig. S6). The bar represents the mean ± s.d
