Fig. 2

Ngζ_1 phosphorylates different UDP-activated sugar species. a High pressure liquid chromatography analysis of small metabolite extracts from E. coli strain C41(DE3) cells expressing either ngζ_1 (red) or the inactive ngζ_1(K115A) variant (blue). Separation of UNAG, UDP-glucose and UNAM phosphorylated by ngζ_1 in vitro are shown for genuine standards (gray). b Mass determination of isolated, phosphorylated UNAG, UDP-glucose and UNAM by ESI-MS