Fig. 4

Effect of ngζ_1 products on enzymes involved in early peptidoglycan synthesis. a Effect of in vitro phosphorylated ngζ_1 products on the activity of the first three enzymes of the peptidoglycan synthesis, MurA (monitoring phosphate release), MurB (monitoring NADPH oxidation) and MurC (monitoring phosphate release). In vitro reactions were perfomed in presence of either the native non-phosphorylated substrate (black; 50 µM UNAG, 30 µM EP-UNAG or 50 µM UNAM), the phosphorylated form (red; 50 µM UNAG-4P, 30 µM EP-UNAG-4P, 50 µM UNAM-4P), or both at equimolar concentrations (blue). Reactions were started upon addition of either 0.25 µM MurA, 50 nM MurB, or 2 µM MurC (time 0 s). b Effect of UNAM and UNAM-4P on MurA activity. MurA activity assay using 100 µM UNAG (black) performed in the presence and absence of additional 100 µM UNAM-4P (blue) or UNAM (red)