Fig. 5
Positioning of HA residue 190 in different H3 strains. a The interaction between the RBS of Wy03 D190E and sialic acid (yellow sticks) is shown. b 293S-expressed recombinant HA was purified and analyzed by sialoside glycan array. HAs in the left column carry a Glu at residue 190. HAs in the right column carry an Asp at residue 190. c, d The orientations of human receptor analog 6′-SLN in complex with HK68 WT, HK68 E190D, Wy03 WT, and Mich14 WT are compared. The orientation of 6′-SLN in complex with Wy03 WT and Mich14 deviates from that with HK68 WT and HK68 E190D. This deviation is indicated by the curved arrow. c Top view of the RBS. d Side view of the RBS. e–h The interactions of 6′-SLN (yellow sticks) with e the RBS of HK68 WT (PDB 2YPG9), f the RBS of HK68 E190D, g the RBS of Wy03 WT, and h the RBS of Mich14 WT are shown. Hydrogen bonds are represented by dashed lines
