Fig. 6 | Nature Communications

Fig. 6

From: A complex epistatic network limits the mutational reversibility in the influenza hemagglutinin receptor-binding site

Fig. 6

Deep mutational scanning of residue 225, 226, and 228. a Log10 RF index of each variant is compared between Vic11 WT and D190E genetic backgrounds. b Effect of different combinations of substitutions on Vic11 replication fitness was examined by virus rescue experiments. Virus titer was measured by TCID50. Error bars indicate the standard deviation of three independent experiments. The green dashed line represents the lower detection limit. c, d Log10 RF index of each variant is compared between c HK68 WT and E190D genetic backgrounds and d Vic11 WT and HK68 WT genetic backgrounds

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