Fig. 6
The mutations selected by FI6v3 increase neutralization resistance, but the effects are small. a Neutralization curves of individual viral mutants with FI6v3. The mutations K280S, K280T, N291S, G47R (HA2), and K(-8)T are all expected to increase neutralization resistance based on the mutational antigenic profiling (Fig. 5a), whereas K280A, M17L (HA2), P80D, and V135T are not expected to affect neutralization (Supplementary Fig 6). All neutralization curves in this panel were performed in triplicate on the same day. This panel shows the average of the replicates; Supplementary Fig. 9 shows the curves for each replicate individually and performs statistical testing of whether the IC50s for mutants are significantly different than for wildtype. b,c In contrast to FI6v3, mutations selected by narrow antibodies have very large effects on neutralization. Neutralization curves for representative escape mutants from H17-L19 and H17-L7 taken from Doud et al.47 are shown. Points indicate mean and standard error of three replicates
