Fig. 7
From: Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis
Crossreactive serum albumin–antibody fusion reverses inflammation in vivo. a Clinical score (% of max) and b change in ankle thickness (mm) of mice treated with serum albumin–antibody fusion proteins on day 0 (preventative regimen). Arthritogenic serum was injected into C57BL/6J on days 0 and 2. Mice were also treated daily with SA129, SA138, and SACTR fusions (1 mg per mouse in PBS i.p.) beginning on day 0. Paw thickness of ten mice per group (n = 10), pooled from two independent experiments, were measured every 2 days for a total of 14 days. Arrows indicate first day of treatment. Data are presented as mean (dots) ± s.e.m. (bars). c Columns graph reporting the number of infiltrating synovial fluid neutrophils (Ly6G+ cells) from the ankles of serum-transferred arthritic mice measured at day 8 by flow cytometry (n = 3 per condition). Statistical comparisons were made between each group using one-way analysis of variance (ANOVA), followed by Tukey’s test to calculate P-values: *P < 0.05, **P < 0.01, ***P < 0.001; ****P < 0.0001. ns: non-significant. d Columns graph reporting the histopathological scoring and e representative H&E staining of ankle tissue sections of mice treated with SA129 (top), SA138 (middle), and control SACTR (bottom) on day 8. Scale bar represents 200 μm. White arrows indicate joint-infiltrating inflammatory cells, and red arrows indicate pannus formation. T taulus, N navicular. f Clinical score (% of max) and g change in ankle thickness (mm) of K/BxN serum-induced arthritic mice treated beginning on day 4 with serum albumin–antibody fusion proteins (therapeutic regimen). Arthritogenic serum was injected into C57BL/6J on days 0 and 2, and mice were then treated daily i.p. with SA129, SA138, and SACTR fusions (1 mg per mouse in PBS i.p.) beginning on day 4 after inflammation had developed. Paw thickness of ten mice per group (n = 10), pooled from two independent experiments, was measured every 2 days for a total of 14 days. Arrows indicate the day treatment began. Data are presented as mean (dots) ± s.e.m. (bars)
