Fig. 3
From: Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons
Widespread CG and localised CH methylation in Symbiodinium. a Percentage of methylated cytosines in distinct sequence contexts in four MethylC-seq libraries. H represents any base but a G. The lambda genome methylation indicates the bisulfite non-conversion rate for each library. b Unimodal distribution of methylation levels (the ratio of C base calls to total base calls) on individual CG sites with high coverage (≥10 reads) in S. kawagutii 25 °C culture sample. c Methylation level of mapped reads with ≥5 CGs in S. kawagutii 25 °C culture sample. d Genome browser display showing the profile of methylation on genes expressed at different levels in a single scaffold. rpm reads per million. e Methylation profiles in CG and CH contexts on S. kawagutii genes and transposable elements. Mean (thick line) and standard deviation (shade) methylation levels for each relative position around genomic features. f Genome browser display demonstrating CH methylation enrichments on 3′ end of silent DNMT encoding retrotransposons found in introns of an expressed gene. g Methylation profiles in CG and CH contexts on S. minutum genes and transposable elements. Line represents mean and pale shade represents standard deviation. TSS transcriptional start site, TES transcriptional end site, TE transposable element
