Fig. 2
Replication of HIV-1-like SIVagm constructs in vivo. a Mean levels of plasma viremia in AGMs infected with the indicated SIVagm constructs. Three animals were infected with each virus and plasma RNA loads were determined as described in the Methods section. Arrows indicate time points used for single genome viral RNA analysis (SGA), determination of proviral copy numbers (vDNA), or microarray analysis (Array). Symbols refer to all panels. The limit of viral RNA detection is ~100 copies/ml of plasma. b Absolute mean CD4+ T cell counts in AGMs infected with the WT, GU, 1N, and GU1N SIVagm constructs. The prevalue (Pre) represents the average CD4+ T cell count of four measurements prior to infection. c Absolute numbers and percentages of CD4+ T cells at later time points of infection (>170 wpi) with mean ± SEM. Each symbol represents one measurement. d Correlation between the average vRNA loads and CD4+ T cell counts (left) and percentages (right) in the final year of follow-up. Each symbol corresponds to one AGM and represents the average value of nine measurements from 171 to 240 wpi. The open triangle represents AGM14627 that was accidentally infected. p-values were calculated using the two-tailed unpaired Student's-t-test and significant differences are indicated as: *p < 0.05; **p < 0.01; ***p < 0.001
