Fig. 1
From: Prokaryotic nanocompartments form synthetic organelles in a eukaryote
Expression, assembly and cargo loading of encapsulins in yeast. a Co-expression of encapsulin and targeted cargo in yeast results in self-assembly of cargo-loaded nanocompartments, with an internal diameter of 26 nm (structure from PDB: 4PT2). Encapsulin compartments can stabilize and co-localize cargo proteins, as well as allow for catalysis to occur within their interior. b Encapsulin EncA (Enc, 32.5 kDa) from Myxococcus xanthus can be purified to homogeneity from yeast, as determined by SDS-PAGE and TEM. Full gel image is shown in Supplementary Fig. 1. Scale bars: zoom out: 400 nm, zoom in: 50 nm. c Heterologous proteins such as mNeonGreen (mNeon, 28.4 kDa) can be packaged inside encapsulin compartments, as determined by SDS-PAGE and TEM on the co-purified sample. Full gel image is shown in Supplementary Fig. 2. M1 = Color Prestained Protein Ladder, Broad Range (11–245 kDa, NEB). Scale bars: zoom out: 200 nm, zoom in: 50 nm
