Fig. 4
IL-6Rα deficiency compromises CCR-6-expressing lymphocyte recruitment in CAC. a IPA analysis diagram of quantity of downregulated (green) and upregulated (red) lymphocyte-specific genes in tumours derived from 17-week-old HFD-fed Il6rαFl versus Il6rαKO mice at day 62 of the 1.5% AOM/DSS protocol. Upper number represent respective p-value, lower number fold change. b qPCR analysis of indicated gene expression in tumours of 17-week-old Il6rαFl versus Il6rαKO (n = 6–12) mice at day 62 after the 1.5% AOM/DSS protocol, results are presented relative to NCD Il6rαFl non-colitic colons. Representative immunofluorescent stainings of c FoxP3 (red), d γδTCR (red) and e IgM (green) expressing cells in colons from 17-week-old HFD-fed Il6rαFl and Il6rαKO mice at day 62 of the 1.5% AOM/DSS protocol counterstained with Hoechst 3342. f Total number of CD45+, F4/80+ and F4/80− cells isolated from non-colitic and colitic colons at day 0 or day 13 of the 1.5% AOM/DSS protocol in 8- and 10-week-old NCD-fed Il6rαFl (n = 4–5) versus Il6rαKO (n = 4) mice. g Total number of CD19+, γδTCR+ and βTCR+ cells isolated from non-colitic and colitic colons at day 0 or day 13 of the 1.5% AOM/DSS protocol in 8 and 10-week-old NCD-fed Il6rαFl (n = 4–5) versus Il6rαKO (n = 4) mice. h Total number of CD19+CCR-6+, γδTCR+CCR-6+ and βTCR+CCR-6+ cell isolated from non-colitic and colitic colons at day 0 or day 13 of the 1.5% AOM/DSS protocol in 8- and 10-week-old NCD fed Il6rαFl (n = 4–5) versus Il6rαKO (n = 4) mice. AOM, azoxymethane; DSS, dextran sodium sulphate; NCD, normal chow diet; HFD, high-fat diet; IPA, Ingenuity pathway analysis; CAC, colitis-associated colorectal cancer. Centre line: median; box limits: 1st and 3rd quartiles; whisker: maximum to minimum, *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001 two-tailed unpaired Student’s t-test b, one-way ANOVA followed by Fisher LSD f–h. Scale bar, 100 μm c, e, 200 μm d
