Fig. 4

RP–CP interactions regulate the gate opening. a Overview of the RP–CP interface in which the C-terminal tails of Rpt1, Rpt2, Rpt6, Rpt3, and Rpt5 are shown to insert into the corresponding α-pockets in the S_D states. Two empty α-pockets are marked by asterisks. b–f Close-up views of each C-terminal tail shown in a stick representation superimposed over their corresponding cryo-EM densities shown in a mesh representation. g–i The Rpt subunits in cartoon representation superimposed with cryo-EM densities showing the vertical positions of each Rpt subunit relative to the α-ring in a gray cartoon representation in the S_D1 (g), S_D2 (h), and S_D3 (i) states