Fig. 1 | Nature Communications

Fig. 1

From: Transposon-modified antigen-specific T lymphocytes for sustained therapeutic protein delivery in vivo

Fig. 1

Vector schematics. a The piggyBac transposase was used with the pT-Tight-hEPO, pT-EF1α-mEPO, and pT-effluc-Thy1.1 transposons. b The Sleeping Beauty transposase was used with the pTSB-CAG-OVA transposon. CMV, cytomegalovirus immediate early enhancer/promoter; piggyBac, transposase; pA, SV40 polyadenylation signal; HS4E, core insulator sequence from the chicken B-globin 5′HS4 element; mEPO, recombinant murine erythropoietin cDNA; hEPO, recombinant human erythropietin cDNA; CAG, CAG synthetic promoter sequence; effluc, enhanced firefly luciferase; Thy1.1, mouse thy1.1 antigen; TRE, tetracycline response element; Tet-ON 3G, tetracycline transactivator; WPRE, woodchuck hepatitis post-transcriptional regulatory element; IRES, internal ribosomal entry site; SV40, simian virus 40 late polyadenylation signal. Solid arrows indicate inverted terminal repeat (ITR) sequences: black, piggyBac ITRs; blue, Sleeping Beauty ITRs

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