Fig. 2
Transposon modification and functional engraftment of OT-1 T cells. CD8+ T cells were modified with the pT-effluc-thy1.1 transposon, and 1 × 107 CD8+ T cells were transferred into host mice. a Representative flow cytometry analysis (from N = 3) showing ~35% of T cells transfected with pT-effluc-thy1.1 plus pCMV-m7pB were thy1.1-positive after 24 h culture post transfection (top) compared to cells transfected with pTSB-CAG-OVA plus pCAG-SB100X (negative control; bottom). b Schematic representation of experimental design. c Representative bioluminescent imaging (N = 5) from a mouse subdermally injected with the pTSB-CAG-OVA plasmid vaccine immediately after adoptive cell transfer (ACT) and on day 10 after ACT. The blue arrow timeline indicates the days after ACT. Red arrows indicate vaccine administration immediately after ACT and on day 10 after ACT. The images a–c correspond to the blue arrow timeline
