Fig. 7

Modified EBV-specific T lymphocytes produce EPO and retain target specificity. a Genome modification of EBV-CTLs with pT-tight-hEPO results in tight regulation of EPO expression (N = 3 ± SEM). b pT-tight-hEPO-modified EBV-CTLs lyse target cells with similar efficiency as unmodified EBV-CTLs, indicating that genome-modified EBV-CTLs retain target specificity. Untransfected EBV-CTLs (UT-EBV-CTLs) lysed autologous LCLs (auto-LCLs) as did EBV-CTLs transfected with pT-tight-hEPO (ihEPO-EBV-CTLs) with auto-LCLs. However, ihEPO-EBV-CTLs did not lyse allologous LCLs (allo-LCLs)