Fig. 2
From: TAp63 contributes to sexual dimorphism in POMC neuron functions and energy homeostasis
TAp63 stimulates POMC gene expression. a Five potential TAp63-binding sites on mouse POMC gene promoter. Site 1: −2011 to −1989; site 2: −1840 to −1818; site 3: −884 to −862; site 4: −155 to −135; site 5: 436–458, relative to the transcriptional start site. b CHIP assay using TAp63 antibody (or IgG as negative controls) to pull down the TAp63-binding sites from mouse hypothalamus. c Effects of TAp63α on POMC-luciferase activity in N46 cells. WT, pGL3b-POMC promoter; D2-4, pGL3b-POMC promoter with the deletion of sites 2, 3, or 4. Data are presented as box and wiskers showing minimal, maximal, and median values with individual data points. N = 3. *P < 0.05 vs. mock in the same POMC promoter; #P < 0.05 vs. WT POMC promoter in response to the same TAp63 overexpression in two-way ANOVA analysis followed by post hoc Sidak tests
