Fig. 2

Proliferation of IL10 + CpG-stimulated and MYC-overexpressing cells is dependent on different glutamine-derived metabolites. a Mean isotopic enrichment of metabolites in unstimulated and IL10 + CpG-stimulated P493-6 MYC^low, as well as unstimulated P493-6 MYC^high cells treated with ¹³C₅-glutamine within the text only 13C5-Gln is used?. b Scheme of oxidative decarboxylation (blue) and reductive carboxylation (red). Both can be distinguished by isotopic labelling in mass spectrometry. c Isotopic fractions of citrate in IL10 + CpG-stimulated P493-6 MYC^low and P493-6 MYC^high cells. Mass spectra were analysed according to the different masses of citrate. Data on 1-¹³C-glutamine within the main text 1-13C-Gln is used ? labeling are summarized in Supplementary Fig. 5. Fractions of d m + 1 ¹⁵N isotopologue in selected amino acids and e ¹⁵N-enriched nucleobases in unstimulated and IL10 + CpG-stimulated P493-6 MYC^low, as well as P493-6 MYC^high cells. f,g Relative cell counts of IL10 + CpG-stimulated P493-6 MYC^low and P493-6 MYC^high cells grown in Gln-deprived and metabolite-treated media. Cells were treated with either f TCA metabolites (Pyr = pyruvate, α-KG = dimethyl 2-oxoglutarate, Mal = diethyl malate, OAA = oxaloacetate) or g aspartate (Asp) and nucleobases (A = adenine, T = thymine) for 24 h. For all plots, error bars represent mean ± SD of three independent experiments and results from Bonferroni post hoc tests on a one-way ANOVA results are given (*p < 0.05, **p < 0.01, ***p < 0.001)