Table 1 Reduction of 3-thiazolines using imine reductases (IREDs)

From: Enantioselective reduction of sulfur-containing cyclic imines through biocatalysis

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  1. a Examined imine reductases (IREDs) are literature-known enzymes22,29; for reasons of clarity, IREDs are numbered throughout this manuscript, and the corresponding names of the original strains with information about the gene sequences are given in Supplementary Table 4 and Supplementary Methods. IRED5: Cupriavidus sp. HPC(L); IRED8: Mycobacterium smegmatis; IRED24: Glycomyces tenuis; total protein concentration (crude extract; for expression, see SDS-PAGE, Supplementary Fig. 39) are given in footnotes d and e
  2. bConversion (conv.) determined by GC
  3. c Enantiomeric excess (ee) was determined by means of chiral SFC-HPLC after derivatization. Absolute configuration was determined by vibrational circular dichroism for (S)-2f and absolute configuration for other 3-thiazolidines was assigned in analogy according to chiral SFC-HPLC data (for details, see Supplementary Table 6 and Supplementary Methods)
  4. d 5 mg mL−1
  5. e 1.2 mg ml−1
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