Fig. 2
From: Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration
H3mm7−/− causes the rate change of gene expression during differentiation. a For all DEGs, the rate change of gene expression was observed throughout the differentiation time course. The illustrations explain the result of the statistical test against RNA-seq data. b The four types of rate changing patterns during differentiation are explained by the combination of the patterns: Up/Down in time and Above/Below in H3mm7−/− cells. The points indicate the cluster average of the relative (gene-wise Z-normalized) expression levels of two cell types (H3mm7+/+ and H3mm7−/−) with two replicates. The error bars indicate ± 1 SD. The numbers of the genes in the clusters are indicated in parentheses. c LDA extracted two major gene expression patterns during differentiation that correspond to A/B and U/D. The points indicate the genes of the clusters in LD space spanned by LD1 (middle) and LD2 (right) components. d The singular values of the LDA (the ratio of between- and within-cluster standard deviations) indicate that the two most relevant components to discriminate the clusters were LD1 (A/B) and LD2 (U/D)
