Fig. 5
Chromatin accessibility changes in cigarette smoke-treated or HDAC11-overexpressed RPE cells. a Flow cytometric analysis of the expression of RPE specific markers RPE65 and RLBP1 from 12-week-old iPSC-derived RPE monolayers. Isotype was used as the control for gating strategy. b Changes in chromatin accessibility after cigarette smoke treatment. The average intensities of two replicates were used in the analysis. The percentage of reduced peaks is shown under the density curve in the right panel. c Comparison of chromatin accessibility changes in AMD RPE and cigarette smoke-treated RPE cells. d HDAC11 expression in peripheral RPE at different disease stages (n = 46 for normal, 9 for pre-AMD, and 14 for AMD). One-way ANOVA test was performed. The red symbol ‘ + ‘ indicates the outlier. The data are from NCBI GEO GSE29801. e HDAC11 and DAPI staining in the RPE cell. Scale bar, 50 µm. f Changes in chromatin accessibility with HDAC11 overexpression. g Comparison of accessibility changes in AMD RPE and HDAC11-overexpressed RPE cells. The gray dashed line is the fitting line. R is Pearson’s correlation coefficient
