Fig. 1
Our multi-omics integrative analysis of the monolignol biosynthetic pathway in P. trichocarpa predicts wood properties for wood utilization. The pathway is represented by 21 monolignol genes (turquoise circles) identified from the genome sequence, and their corresponding transcripts (blue circles) and proteins (red circles). The gene transcript abundances can be converted to protein abundances by specific βi values from linear regression models (Supplementary Table 2). The monolignol proteins in a metabolic grid convert phenylalanine to monolignols in stem-differentiating xylem. The metabolic grid consists of 24 metabolites (underlined numbers within structures), 37 reaction fluxes (white numbers on black circles), 104 reaction kinetic parameters and 103 inhibition kinetic parameters. Other enzymes, regulators, and components are involved but genes encoding these factors are unknown or not yet sufficiently characterized to be included in this study. Fluxes 16, 20, 21, and 26 were set to zero because kinetic parameters for these reactions are not currently available. Colored lines represent pathway enzyme inhibitions. Multiple linear regressions predict the lignin and wood properties. Abbreviations: Reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxidized nicotinamide adenine dinucleotide phosphate (NADP+), coenzyme A (CoA), adenosine triphosphate (ATP), adenosine monophosphate (AMP), pyrophosphate (PPi), S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH)
