Fig. 2
Phospho-S265 JMJD1A induces beige biogenesis. a qPCR analysis demonstrates decreased expression of beige-selective genes in scWAT from Jmjd1a-S265AKI/KI mice exposed to 30 °C or 4 °C for 1 week (n = 5 per genotype group). b Immunoblot analysis of UCP1 and PPARγ in tissue homogenates of scWAT from mice presented in a. Uncropped images of the blots are shown in Supplementary Fig. 8. c Haematoxylin and eosin (H&E) and UCP1 staining sections of scWAT from WT and Jmjd1a-S265AKI/KI mice exposed to chronic cold exposure (4 °C for 1 week) (scale bar, 100 μm). d NE-induced oxygen consumption rate (OCR) in mice exposed to chronic cold exposure (4 °C for 4 weeks) (n = 7 per genotype group) (left). OCR before and 30 min after NE treatment are analyzed (right) (n = 7). e OCR of scWAT from mice exposed to 30 °C or 4 °C for 1 week (WT: n = 3; Jmjd1a-S265AKI/KI: n = 4). Data are mean ± s.e.m. a, d, e Analysis of variance were performed followed by Tukey’s post hoc comparison in a. Student’s t test was performed for comparisons in d, e. *P < 0.05, **P < 0.01, and ***P < 0.005 were considered statistically significant
