Fig. 2
Glucose influences the interactions between BRI1 and BAK1. a Glucose regulates the physical interactions between BRI1 and BAK1. pBRI1:BRI1-GFP;35S:BAK1-HA or 35S:BAK1-HA seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. b Quantification of BRI1-GFP and BAK1-HA associations in Arabidopsis seedlings treated with different concentrations of glucose (G) for 4 h from a. The ratio value of immunoprecipitated BAK1-HA to input BAK1-HA in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD (n = 3). **P < 0.01 compared with the value for 0% glucose treatment by Student’s t-test. c Glucose influences the phosphorylation levels of BRI1 in Arabidopsis. pBRI1:BRI1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN input; IP immunoprecipitation; IB immunoblot. d Quantification of BRI1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from c. The ratio value of phosphorylated BRI1-GFP to immunoprecipitated BRI1-GFP in response to 0% glucose was set at 1. Values for glucose treatments are given as mean ± SD (n = 3). **P < 0.01 compared with the value for 0% glucose treatment by Student’s t-test. e Glucose influences the phosphorylation levels of BAK1 in Arabidopsis. pBAK1:BAK1-GFP seedlings were grown in the light for 5 days, then incubated in darkness for 4 days, and treated with different concentrations of glucose (G) for 4 or 24 h. IN, input; IP immunoprecipitation; IB immunoblot. f Quantification of BAK1-GFP phosphorylation in Arabidopsis seedlings treated with different concentrations of glucose for 4 h from e. The ratio value of phosphorylated BAK1-GFP to immunoprecipitated BAK1-GFP was set at 1. Values for glucose treatments are given as mean ± SD (n = 3). **P < 0.01 compared with the value for 0% glucose treatment by Student’s t-test
