Fig. 7
Chemogenetic inhibition of NAc D1R neurons increases NREM sleep and promotes nest-building. a Drawings of superimposed AAV injection sites in the NAc of D1R-Cre mice (n = 9, indicated with different colors). b Representative traces showing that CNO decreased the number of evoked action potentials in an hM4Di-expressing neuron. c Time course of wakefulness, NREM sleep, and REM sleep after administration of vehicle or CNO at 09:00 (n = 9, repeated-measures ANOVA; F1,16 = 26.7 (wake), 24.9 (NREM), 0.3 (REM); P = 8 × 10−5 (wake), 1 × 10−4 (NREM), 0.6 (REM)). d Total amounts of each stage for 3 h following treatments during the light period (n = 9, paired t test; t8 = 7.2, P = 9 × 10−5 (wake); t8 = 6.2, P = 3 × 10−4 (NREM); t8 = 0.5, P = 0.6 (REM)). e EEG power density of NREM and REM sleep after CNO and vehicle injections. Red lines represent P < 0.05 (n = 9, paired t test). f Total amounts of each stage for 3 h following treatments during the dark period (n = 9, paired t test; t8 = 2.9, P = 0.02 (wake); t8 = 2.9, P = 0.02 (NREM); t8 = 1.9, P = 0.09 (REM)). g EEG power density of NREM and REM sleep after CNO and vehicle injections (n = 9, P > 0.05, paired t test). h Representative pictures of hM4Di mouse cages following vehicle and CNO administrations at the end of the 3 h test. i Nesting score during the 3 h test (1, poor; 5, good). Wilcoxon matched-pairs signed rank test, mCherry: n = 8, Z = 0.4, P = 0.7; hM4Di: n = 8, Z = 2.0, P = 0.047. j Inhibition of NAc D1R neurons did not affect food intake (n = 8 for hM4Di group and n = 6 for control group, paired t test; 2 h, mCherry: P = 0.8, hM4Di: P = 0.2; 24 h, mCherry: P = 0.5, hM4Di: P = 0.7). Data represent mean ± s.e.m. *P < 0.05, **P < 0.01, NS not significant
