Fig. 8
Chemogenetic inhibition of NAc D2R neurons increases wakefulness, while activation induces NREM sleep in D2R-Cre mice. a Drawings of superimposed AAV-DIO-hM4Di injection sites in the NAc of D2R-Cre mice (n = 6, indicated with different colors). b Representative image of hM4Di-mCherry expression in the NAc. Scale bar: 500 μm. c CNO applied to the bath reduced firing rate in response to 250 pA current injection in an hM4Di-expressing D2R neuron of brain slice. d Typical examples of compressed spectral array EEG, EMG, and hypnograms over 6 h following administration of vehicle or CNO in a D2R-Cre mouse with bilateral hM4Di receptor expression in the NAc. e Time course of wakefulness, NREM sleep, and REM sleep following injection of vehicle or CNO in mice expressing hM4Di receptor in NAc D2R neurons (n = 6, repeated-measures ANOVA; F1, 10 = 16.8 (wake), 15.9 (NREM), 14.6 (REM); P = 0.002 (wake), 0.003 (NREM), 0.003 (REM)). f Total time spent in each stage for 2 h after vehicle or CNO injection (n = 6, paired t test; P = 0.009 (wake), P = 0.01 (NREM), P = 0.012 (REM)). g Drawings of superimposed AAV-DIO-hM3Dq injection sites in the NAc of D2R-Cre mice (n = 5). h Representative image of hM3Dq-mCherry expression in the NAc. Scale bar: 500 μm. i CNO applied to the bath increased firing rate in response to 180 pA current injection in an hM3Dq-expressing D2R neuron of brain slice. j Typical examples of compressed spectral array EEG, EMG, and hypnograms over 6 h following administration of vehicle or CNO in a D2R-Cre mouse with bilateral hM3Dq receptor expression in the NAc. k Time course of wakefulness, NREM sleep, and REM sleep after administration of vehicle or CNO to mice expressing hM3Dq in NAc D2R neurons (n = 5, repeated-measures ANOVA; F1,8 = 17.8 (wake), 16.9 (NREM), 7.6 (REM); P = 0.003 (wake), 0.003 (NREM), 0.025 (REM)). l Total time spent in each stage for 2 h after vehicle or CNO injection (n = 5, paired t test; P = 0.003 (wake), P = 0.002 (NREM), P = 0.1 (REM)). *P < 0.05, **P < 0.01
