Fig. 6
From: A proteomics landscape of circadian clock in mouse liver
The diurnal rhythm of the Mediator complex. a Hierarchical clustering of the proteins ordered by the phase of the oscillation. Values for each protein at all analyzed samples (columns) are color code based on the intensities, low (blue) and high (yellow) z-scored normalized iBAQ. The upper white to black bar indicates the 2 days’ cycle. Daytime is shown in white, while nighttime is shown in black. b Modular structure of Mediator complex recruited by diurnal rhythmic TFs that regulated different bioprocess, subunits colored in yellow or green represent subunits’ high or low DNA-binding activity, respectively. c The regulation network of Mediators, TFs, and TGs in four major pathways. For each pathway, the network on the left shows the TFs recruited components of Mediator to regulate TGs’ expression, proteins (TFs, components of Mediator, TGs) were colored based on their abundance (proteins peaked in the daytime are yellow, in the nighttime are green), and diurnal rhythmic ones were shown with red border (JTK_CYCLE p < 0.1). The upright box plot shows the correlation between pathway-specific TFs with Mediators is higher than the average correlation between Mediators with total TFs (pair tailed Student’s t test p < 0.05). For the box plot, the bottom and top of the box are the first and third quartiles, and the band inside the box is the median of the correlation between TFs and Mediators. The Heat map on the down-right shows the diurnal difference of the correlation between pathway-specific TFs with Mediators (pair tailed Student’s t test p < 0.05)
