Fig. 7

The diurnal switch of the immune response in mouse liver. a Systematic workflow of the diurnal KC sub-proteome. b Diurnal oscillation of the number of KCs during the circadian cycle. c Bar plot shows distribution of the diurnal oscillated proteins of KCs at each ZT point. d Venn plot shows the overlap among diurnal rhythmic whole-liver proteins (JTK_CYCLE p < 0.1) and diurnal rhythmic KC proteins (JTK_CYCLE p < 0.1). e Scatterplot shows statistically enriched GO/KEGG pathways by diurnal rhythmic KC proteins and diurnal rhythmic whole-liver proteins. f Box plots shows the pathway-specific KC proteins’ abundance of expression at ZT0 versus their expression at ZT12 (pair tailed Student’s t test p < 0.05). For the box plot, the bottom and top of the box are the first and third quartiles, and the band inside the box is the median of the proteins’ abundance. g Schematic representation of diurnal changed TLR pathway-related proteins; for each protein, the corresponding expression level was represented by color. The color bar indicates normalized z-scored iBAQ. h Box plots show the stronger connection (more interactions) among immune-related KC proteins and whole-liver proteins in the daytime and stronger connection (more interactions) among lipid-related KC proteins and whole-liver proteins in the nighttime (pair tailed Student’s t test p < 0.05). For the box plot, the bottom and top of the box are the first and third quartiles, and the band inside the box is the median of the interactions between KC proteins and whole-liver proteins. i Diurnal interaction network among KC proteins and whole-liver proteins. KC upregulated proteins were colored in green, whole-liver upregulated proteins were colored in red. Diurnal rhythmic proteins (whole-liver proteins: JTK_CYCLE p < 0.1, KC proteins: JTK_CYCLE p < 0.1) were shown with red border, diurnal changed KC proteins (fold change > 5) were shown with black border