Table 1 Results of sequencing sgRNA PCR products from talazoparib-resistant mouse ES cell clones isolated from the CRISPR screen described in Fig. 1a
Clone | Likely cause of resistance | Clonal with | sgRNAs identified |
|---|---|---|---|
BR1 | Parp1 | 7 | Parp1 (#1), Tmed5 |
BR2 | Parp1 | — | Parp1 (#1) |
BR3 | — | Gpd2 | |
BR4 | — | Tbx20, Gpr89, Pde4d, Arhgap12 | |
BR5 | 21 | Trp53, Csmd1 | |
BR6 | Parp1 | — | Parp1 (#2), Apol6 |
BR7 | Parp1 | 12,1 | Parp1 (#1), Abca14, Dtnb, Tmed5 |
BR8 | Parp1 #2 | — | Parp1 (#2), Lemd1, Slmo1 |
BR9 | Parp1 #2 | — | Parp1 (#2) |
BR10 | Parp1 | — | Parp1 (#1), Spock1, P2ry6, AW209491 |
BR11 | Tdg | 14 | Tdg, Gm5597 |
BR12 | Parp1 (by clonality) | 7 | Abca14, Dtnb |
BR13 | Parp1 | — | Parp1 (#1), Nwd1, Scn10a |
BR14 | Tdg | 11 | Tdg, Gm5597, Tmed5 |
BR15 | — | ND | |
BR16 | — | Defb25 | |
BR17 | — | ND | |
BR18 | — | Traf3ip1, Hkdc1, Gm4876 | |
BR19 | — | Dnajb11, Scaf8, Acbd3, Msh2 | |
BR20 | — | ND | |
BR21 | 5 | Trp53, Csmd3, Chst15 | |
BR22 | — | Cyp2r1, Tmem69 | |
BR23 | — | ND | |
BR24 | Tdg | — | Tdg |
