Fig. 2
From: Cathelicidins prime platelets to mediate arterial thrombosis and tissue inflammation
Cathelicidins contribute to arterial thrombosis. a–d Ferric chloride induced thrombus formation in the mouse carotid artery. a Representative intravital microscopy images of wild type (wt → wt) and CRAMP-deficient (ko → wt) BM chimeras. Thrombus size was visualized by in vivo staining with DiOC6. Bar, 400 µm. Analysis of b time to complete thrombotic occlusion of the carotid artery, c maximal thrombus size, and d duration of vessel occlusion (n = 6). e, f Platelet recruitment and aggregate formation after carotid artery injury induced by temporary mechanical ligation in wild type (wt → wt) and CRAMP-deficient (ko → wt) BM chimeras. Platelets were labeled in vivo using a DyLight488-labeled non-blocking GPIbβ antibody. e Representative intravital microscopy images. Bar, 50 µm. f Quantitative analysis of platelet recruitment by measuring mean fluorescence intensity (MFI) at the site of injury versus background fluorescence (n = 4). g, h Hemostatic parameters. g Tail bleeding time (n = 4). h Clotting time and clot formation time induced by either extrinsic or intrinsic activation of coagulation (n = 6). Graphs show mean and SEM. All P-values were determined by unpaired t-test except for h (extrinsic clotting time, Mann–Whitney U-test)
