Fig. 3

BL001 promotes a pancreatic anti-inflammatory/tolerogenic environment. RIP-B7.1 male and female mice were pre-treated or not with BL001 for 5 days and then immunized. Treatment with BL001 was pursued for an additional 8 weeks at which point animals were sacrificed and pancreas extracted and processed for flow cytometry quantification of a Tregs (n = 10–16 mice), b Th1 (n = 5–11 mice), c Th2 (n = 5–11 mice), d Th17 (n = 5–11 mice), e dentritic cells (n = 5 mice), f macrophages (n = 7 mice), and g M2-like macrophages (n = 7 mice). h Heatmap displaying expression values of a subset of M2 macrophage genes in F4/80⁺/CD11b⁺/CD206⁺ pancreatic subpopulations isolated from immunized mice treated or nor with BL001 (IMN and IMN BL). Also depicted are raw p-values and the graphical representation of logFC from the differential expression analysis (IMN BL versus IMN). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001, one-way ANOVA (a–g)