Fig. 3 | Nature Communications

Fig. 3

From: Tamoxifen-resistant breast cancer cells are resistant to DNA-damaging chemotherapy because of upregulated BARD1 and BRCA1

Fig. 3

Reduced BARD1 or BRCA1 expression sensitizes tamoxifen-resistant cells to cisplatin. MCF7-Re cells were transfected with negative control siRNA (nc), BARD1, and/or BRCA1 siRNAs. a, b Cell viability assay showing the sensitivity to cisplatin (a) and ADM (b). c Apoptosis assay by AnnexinV-FITC/PI staining showing the effect of cisplatin on the percentages of apoptotic cells. d The expression of γH2AX induced by cisplatin. e Comet assay showing the DNA damage induced by cisplatin. Scale bar indicated 100 μm. In a–c, e, data show means ± s.d. (n = 3) **p < 0.01 compared with control by Student’s t test

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