Fig. 7
From: TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC progression
miR-100 and miR-125b target similar transcripts to regulate common pathways. a, b Venn diagrams showing miR-125b (a) or miR-100 (b) direct targets represented as overlap of the top 4000 transcripts most enriched onto AGO2 from RIP-seq (RIP) and the top 1300 down-regulated transcripts in RNA-seq (DW), after miRNA overexpression. The bar charts indicate the statistical significance of the overlap between the two groups. P-values were calculated using exact hypergeometric probability test. c, d IPA shows the most enriched canonical pathways for miR-125b (c) or miR-100 (d). Genes from the overlap between RIP and DW were used as input for the analysis. The threshold of significance is indicated by the intermitted line. Positive Z-score (red) indicates that the canonical pathway is activated and negative Z-score (blue) that is inhibited, based on the expression values in the data set. e, f Sub-networks of genes belonging to significantly enriched IPA canonical pathways using the gene signature coming from overlap between RIP and DW regulated by miR-125b (e) or miR-100 (f). MiRNA–target interactions inferred from RIP-USE are depicted by different line. Node color represents change in gene expression, from the RNA-seq, mediated by the overexpression of each miRNA
