Table 1 Oligonucleotides for sgRNA cloning
From: TGF-β induces miR-100 and miR-125b but blocks let-7a through LIN28B controlling PDAC progression
sgRNA | Sequence |
|---|---|
mir-100 sgRNA1-F | CACCGATCTACGGGTTTGTGGCAAC |
mir-100 sgRNA1-R | AAACGTTGCCACAAACCCGTAGATC |
mir-100 sgRNA2-F | CACCGTTAGGCAATCTCACGGACC |
mir-100 sgRNA2-R | AAACGGTCCGTGAGATTGCCTAAC |
mir-125b sgRNA1-F | CACCGACCGTTTAAATCCACGGGTT |
mir-125b sgRNA1-R | AAACAACCCGTGGATTTAAACGGTC |
mir-125b sgRNA2-F | CACCGCGAGTCGTGCTTTTGCATCC |
mir-125b sgRNA2-R | AAACGGATGCAAAAGCACGACTCGC |
MIR100HG∆P sgRNA1-F | CACCGCAAGAGTGTAAAGACCCCGA |
MIR100HG∆P sgRNA1-R | AAACTCGGGGTCTTTACACTCTTGC |
MIR100HG∆P sgRNA2-F | CACCGGAGCATACGTGTCCCCATC |
MIR100HG∆P sgRNA2-R | AAACGATGGGGACACGTATGCTCC |
LIN28B sgRNA-F | CACCGCCGTGGGGCAACATGGCCGA |
LIN28bB sgRNA-R | AAACTCGGCCATGTTGCCCCACGGC |
