Fig. 3
From: Cell-free prediction of protein expression costs for growing cells
Predicting the burden of operon designs for the Luciferase biosynthesis pathway. a Diagram of the luciferase pathway and the γ-values for the two enzyme-encoding sequences as measured by the cell lysate capacity assay (see Supplementary Fig. 5). The operon is designed with randomised RBS sequences and promoter BBa_J23100 (strong). b Model-predicted burden of each operon design compared to the measured capacity of E. coli expressing the operons. d-Luciferin and d-cysteine are added to the media to enable luminescence measurements and these are represented by the red intensity in each circle. Error bars show standard error of three independent repeats. Values are normalised to the capacity obtained with capacity monitor plasmid alone
